Xyloglucan-specific endo-beta-1,4-glucanase explained
| Xyloglucan-specific endo-beta-1,4-glucanase |
| Ec Number: | 3.2.1.151 |
In enzymology, a xyloglucan-specific endo-beta-1,4-glucanase is an enzyme that catalyzes the chemical reaction
xyloglucan + H2O
xyloglucan oligosaccharides
Thus, the two substrates of this enzyme are xyloglucan and H2O, whereas its product is xyloglucan oligosaccharides.
This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase. Other names in common use include XEG, xyloglucan endo-beta-1,4-glucanase, xyloglucanase, xyloglucanendohydrolase, XH, and 1,4-beta-D-glucan glucanohydrolase.
Family 12 was first identified in plant pathogens by discovery in Phytophthora spp.
Structural studies
As of late 2007, 15 structures have been solved for this class of enzymes, with PDB accession codes,,,,,,,,,,,,,, and .
Further reading
- Pauly M, Andersen LN, Kauppinen S, Kofod LV, York WS, Albersheim P, Darvill A . A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme . Glycobiology . 9 . 1 . 93–100 . January 1999 . 9884411 . 10.1093/glycob/9.1.93 . free .
- Grishutin SG, Gusakov AV, Markov AV, Ustinov BB, Semenova MV, Sinitsyn AP . Specific xyloglucanases as a new class of polysaccharide-degrading enzymes . Biochimica et Biophysica Acta (BBA) - General Subjects . 1674 . 3 . 268–81 . November 2004 . 15541296 . 10.1016/j.bbagen.2004.07.001 .
- Costanzo S, Ospina-Giraldo MD, Deahl KL, Baker CJ, Jones RW . Gene duplication event in family 12 glycosyl hydrolase from Phytophthora spp . Fungal Genetics and Biology . 43 . 10 . 707–14 . October 2006 . 16784880 . 10.1016/j.fgb.2006.04.006 .
