Vibriolysin Explained

Vibriolysin
Ec Number:	3.4.24.25
Cas Number:	69598-88-5

Vibriolysin (Aeromonas proteolytica neutral proteinase, aeromonolysin) is an enzyme.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

Preferential cleavage of bonds with bulky hydrophobic groups in P2 and P1'. Phe at P1' is the most favoured residue, which distinguished this enzyme from thermolysin

This thermostable enzyme is isolated from Vibrio proteolyticus.

Notes and References

1. Holmquist B, Vallee BL . Esterase activity of zinc neutral proteases . Biochemistry . 15 . 1 . 101–7 . January 1976 . 2276 . 10.1021/bi00646a016 .
2. Book: Wilkes SH, Prescott JM . Aeromonas neutral protease . Part B: Proteolytic Enzymes . Methods in Enzymology . 45 . 404–15 . 1976 . 1012006 . 10.1016/s0076-6879(76)45036-x . 978-0-12-181945-3 .
3. Bayliss ME, Wilkes SH, Prescott JM . Aeromonas neutral protease: specificity toward extended substrates . Archives of Biochemistry and Biophysics . 204 . 1 . 214–9 . October 1980 . 7000005 . 10.1016/0003-9861(80)90026-0 . free .
4. Wilkes SH, Bayliss ME, Prescott JM . Critical ionizing groups in Aeromonas neutral protease . The Journal of Biological Chemistry . 263 . 4 . 1821–5 . February 1988 . 10.1016/S0021-9258(19)77950-2 . free . 3123480 .
5. David VA, Deutch AH, Sloma A, Pawlyk D, Ally A, Durham DR . Cloning, sequencing and expression of the gene encoding the extracellular neutral protease, vibriolysin, of Vibrio proteolyticus . Gene . 112 . 1 . 107–12 . March 1992 . 1551587 . 10.1016/0378-1119(92)90310-l .