Unspecific peroxygenase explained

Unspecific peroxygenase
Ec Number:	1.11.2.1

Unspecific peroxygenase (aromatic peroxygenase, mushroom peroxygenase, haloperoxidase-peroxygenase, Agrocybe aegerita peroxidase) is an enzyme with systematic name substrate:hydrogen peroxide oxidoreductase (RH-hydroxylating or -epoxidising).^{[1] [2] [3] [4] [5] [6] [7] [8] [9]} This enzyme catalyses the following chemical reaction

RH + H₂O₂

\rightleftharpoons ROH + H₂O

Unspecific peroxygenase is a heme-thiolate protein comparable to Cytochrome P450 in the ability to catalyze a variety of P450 reactions (hence "unspecific"), but forms a unique, solely fungal, protein family of extracellular enzymes.

Notes and References

1. Ullrich R, Nüske J, Scheibner K, Spantzel J, Hofrichter M . Novel haloperoxidase from the agaric basidiomycete Agrocybe aegerita oxidizes aryl alcohols and aldehydes . Applied and Environmental Microbiology . 70 . 8 . 4575–81 . August 2004 . 15294788 . 492325 . 10.1128/AEM.70.8.4575-4581.2004 .
2. Ullrich R, Hofrichter M . The haloperoxidase of the agaric fungus Agrocybe aegerita hydroxylates toluene and naphthalene . FEBS Letters . 579 . 27 . 6247–50 . November 2005 . 16253244 . 10.1016/j.febslet.2005.10.014 . free .
3. Anh DH, Ullrich R, Benndorf D, Svatos A, Muck A, Hofrichter M . The coprophilous mushroom Coprinus radians secretes a haloperoxidase that catalyzes aromatic peroxygenation . Applied and Environmental Microbiology . 73 . 17 . 5477–85 . September 2007 . 17601809 . 2042081 . 10.1128/AEM.00026-07 .
4. Ullrich R, Dolge C, Kluge M, Hofrichter M . Pyridine as novel substrate for regioselective oxygenation with aromatic peroxygenase from Agrocybe aegerita . FEBS Letters . 582 . 29 . 4100–6 . December 2008 . 19022254 . 10.1016/j.febslet.2008.11.006 . 28889201 . free .
5. Aranda E, Kinne M, Kluge M, Ullrich R, Hofrichter M . Conversion of dibenzothiophene by the mushrooms Agrocybe aegerita and Coprinellus radians and their extracellular peroxygenases . Applied Microbiology and Biotechnology . 82 . 6 . 1057–66 . April 2009 . 19039585 . 10.1007/s00253-008-1778-6 . 28195958 .
6. Kinne M, Poraj-Kobielska M, Aranda E, Ullrich R, Hammel KE, Scheibner K, Hofrichter M . Regioselective preparation of 5-hydroxypropranolol and 4'-hydroxydiclofenac with a fungal peroxygenase . Bioorganic & Medicinal Chemistry Letters . 19 . 11 . 3085–7 . June 2009 . 19394224 . 10.1016/j.bmcl.2009.04.015 .
7. Kluge M, Ullrich R, Dolge C, Scheibner K, Hofrichter M . Hydroxylation of naphthalene by aromatic peroxygenase from Agrocybe aegerita proceeds via oxygen transfer from H2O2 and intermediary epoxidation . Applied Microbiology and Biotechnology . 81 . 6 . 1071–6 . January 2009 . 18815784 . 10.1007/s00253-008-1704-y . 28759836 .
8. Kinne M, Poraj-Kobielska M, Ralph SA, Ullrich R, Hofrichter M, Hammel KE . Oxidative cleavage of diverse ethers by an extracellular fungal peroxygenase . The Journal of Biological Chemistry . 284 . 43 . 29343–9 . October 2009 . 19713216 . 2785565 . 10.1074/jbc.M109.040857 . free .
9. Pecyna MJ, Ullrich R, Bittner B, Clemens A, Scheibner K, Schubert R, Hofrichter M . Molecular characterization of aromatic peroxygenase from Agrocybe aegerita . Applied Microbiology and Biotechnology . 84 . 5 . 885–97 . October 2009 . 19434406 . 10.1007/s00253-009-2000-1 . 13141260 .