N-hydroxythioamide S-beta-glucosyltransferase explained

thiohydroximate beta-D-glucosyltransferase
Ec Number:	2.4.1.195
Cas Number:	9068-14-8
Go Code:	0047251

In enzymology, a N-hydroxythioamide S-beta-glucosyltransferase is an enzyme that catalyzes the chemical reaction

UDP-glucose + N-hydroxy-2-phenylethanethioamide

\rightleftharpoons UDP + desulfoglucotropeolin

Thus, the two substrates of this enzyme are UDP-glucose and N-hydroxy-2-phenylethanethioamide, whereas its two products are UDP and desulfoglucotropeolin.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucose:N-hydroxy-2-phenylethanethioamide S-beta-D-glucosyltransferase. Other names in common use include desulfoglucosinolate-uridine diphosphate glucosyltransferase, uridine diphosphoglucose-thiohydroximate glucosyltransferase, thiohydroximate beta-D-glucosyltransferase, UDPG:thiohydroximate glucosyltransferase, thiohydroximate S-glucosyltransferase, thiohydroximate glucosyltransferase, and UDP-glucose:thiohydroximate S-beta-D-glucosyltransferase.

References

• Jain JC, Reed DW, GrootWassink JW, Underhill EW . 1989 . A radioassay of enzymes catalyzing the glucosylation and sulfation steps of glucosinolate biosynthesis in Brassica species . Anal. Biochem. . 178 . 137 - 40 . 2524977 . 10.1016/0003-2697(89)90369-2 . 1 .
• Reed DW, Davin L, Jain JC, Deluca V, Nelson L, Underhill EW . 1993 . Purification and properties of UDP-glucose:thiohydroximate glucosyltransferase from Brassica napus L. seedlings . Arch. Biochem. Biophys. . 305 . 526 - 32 . 8373190 . 10.1006/abbi.1993.1456 . 2 .
• Fahey JW, Zalcmann AT, Talalay P . 2001 . The chemical diversity and distribution of glucosinolates and isothiocyanates among plants . . 56 . 5 - 51 . 11198818 . 10.1016/S0031-9422(00)00316-2 . 1 .
• TF . Abel S . 2004 . Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis . Plant J. . 40 . 893 - 908 . 10.1111/j.1365-313X.2004.02261.x . 15584955 . 6 . 10.1.1.538.667 .