Thermotolerance Explained

Thermotolerance is the ability of an organism to survive high temperatures. An organism's natural tolerance of heat is their basal thermotolerance.^[1] Meanwhile, acquired thermotolerance is defined as an enhanced level of thermotolerance after exposure to a heat stress.^[2]

In plants

See main article: Heat shock response. Multiple factors contribute to thermotolerance including signaling molecules like abscisic acid, salicylic acid, and pathways like the ethylene signaling pathway and heat stress response pathway.^[3]

The various heat stress response pathways enhance thermotolerance.^[4] The heat stress response in plants is mediated by heat shock transcription factors (HSF) and is well conserved across eukaryotes. HSFs are essential in plants’ ability to both sense and respond to stress.^[5] The HSFs, which are divided into three families (A, B, and C), encode the expression of heat shock proteins (HSP). Past studies have found that transcriptional activators HsfA1 and HsfB1 are the main positive regulators of heat stress response genes in Arabidopsis thaliana.^[6] The general pathway to thermotolerance is characterized by sensing of heat stress, activation of HSFs, upregulation of heat response, and return to the non-stressed state.^[7]

In 2011, while studying heat stress A. thaliana, Ikeda et al. concluded that the early response is regulated by HsfA1 and the extended response is regulated by HsfA2. They used RT-PCR to analyze the expression of HS-inducible genes of mutant (ectopic and nonfunctional HsfB1) and wild type plants. Plants with mutant HsfB1 had lower acquired thermotolerance, based on both lower expression of heat stress genes and visibly altered phenotypes. With these results they concluded that class A HSFs positively regulated the heat stress response while class B HSFs repressed the expression of HSF genes. Therefore, both were necessary for plants to return to non-stressed conditions and acquired thermotolerance.^[8]

Notes and References

1. Bokszczanin . Kamila . Fragkostefanakis . Sotirios . Bostan . Hamed . Bovy . Arnaud . Chaturvedi . Palak . Chiusano . Maria . Firon . Nurit . Iannacone . Rina . Jegadeesan . Sridharan . Klaczynskid . Krzysztof . Li . Hanjing . 2013 . Perspectives on deciphering mechanisms underlying plant heat stress response and thermotolerance . Frontiers in Plant Science . 4 . 315 . 10.3389/fpls.2013.00315 . 23986766 . 3750488 . 1664-462X. free .
2. 1991-08-19 . Acquisition of thermotolerance in Saccharomyces cerevisiae without heat shock protein hsp104 and in the absence of protein synthesis . FEBS Letters . en . 288 . 1–2 . 86–90 . 10.1016/0014-5793(91)81008-V . 0014-5793. De Virgilio . Claudio . Piper . Peter . Boller . Thomas . Wiemken . Andres . 1831771 . 25550858 . free .
3. Larkindale . Jane . Hall . Jennifer D. . Knight . Marc R. . Vierling . Elizabeth . 2005 . Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance . Plant Physiology . 138 . 2 . 882–897 . 10.1104/pp.105.062257 . 4629891 . 15923322 . 1150405 . 0032-0889.
4. Sarkar . S. . Islam . A.K.M.Aminul . Barma . N.C.D. . Ahmed . J.U. . Tolerance mechanisms for breeding wheat against heat stress: A review . South African Journal of Botany . May 2021 . 262-277 . 10.1016/j.sajb.2021.01.003. free .
5. Liu . Hsiang-chin . Charng . Yee-yung . 2012-05-01 . Acquired thermotolerance independent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response . Plant Signaling & Behavior . 7 . 5 . 547–550 . 10.4161/psb.19803 . 3419016 . 22516818. 2012PlSiB...7..547L .
6. Yoshida . Takumi . Ohama . Naohiko . Nakajima . Jun . Kidokoro . Satoshi . Mizoi . Junya . Nakashima . Kazuo . Maruyama . Kyonoshin . Kim . Jong-Myong . Seki . Motoaki . Todaka . Daisuke . Osakabe . Yuriko . 2011-12-01 . Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression . Molecular Genetics and Genomics . en . 286 . 5 . 321–332 . 10.1007/s00438-011-0647-7 . 21931939 . 8284912 . 1617-4623.
7. Bäurle . Isabel . 2016 . Plant Heat Adaptation: priming in response to heat stress . F1000Research . 5 . F1000 Faculty Rev–694 . 10.12688/f1000research.7526.1 . 2046-1402 . 4837978 . 27134736 . free .
8. Ikeda . Miho . Mitsuda . Nobutaka . Ohme-Takagi . Masaru . 2011-11-01 . Arabidopsis HsfB1 and HsfB2b Act as Repressors of the Expression of Heat-Inducible Hsfs But Positively Regulate the Acquired Thermotolerance . Plant Physiology . 157 . 3 . 1243–1254 . 10.1104/pp.111.179036 . 0032-0889 . 3252156 . 21908690.