Tetraacyldisaccharide 4'-kinase explained

Symbol:	LpxK
LpxK
Pfam:	PF02606
Pfam Clan:	CL0023
Interpro:	IPR003758
Opm Family:	124
Opm Protein:	4ehx

tetraacyldisaccharide 4'-kinase
Ec Number:	2.7.1.130
Cas Number:	107309-06-8
Go Code:	0009029

Tetraacyldisaccharide 4'-kinase is an enzyme that phosphorylates the 4'-position of a tetraacyldisaccharide 1-phosphate precursor (DS-1-P) of lipopolysaccharide lipid A. This lipid forms outer membranes of Gram-negative bacteria.^[1] This enzyme catalyzes the chemical reaction

ATP + [2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl]-(1->6)-[2- N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate]

\rightleftharpoons

ADP + [2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D- glucosaminyl]-(1->6)-[2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D- glucosaminyl phosphate]

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor.

References

Ray BL, Raetz CR . 1987 . The biosynthesis of gram-negative endotoxin. A novel kinase in Escherichia coli membranes that incorporates the 4'-phosphate of lipid A . J. Biol. Chem. . 262 . 1122 - 8 . 3027079 . 3 .

Notes and References

Garrett TA, Que NL, Raetz CR . Accumulation of a lipid A precursor lacking the 4'-phosphate following inactivation of the Escherichia coli lpxK gene . J. Biol. Chem. . 273 . 20 . 12457–65 . May 1998 . 9575203 . 10.1074/jbc.273.20.12457. free .