Taxadiene synthase explained
| taxadiene synthase |
| Ec Number: | 4.2.3.17 |
| Cas Number: | 169277-52-5 |
| Go Code: | 0050553 |
The enzyme taxadiene synthase (EC 4.2.3.17) catalyzes the chemical reaction
geranylgeranyl diphosphate
taxa-4,11-diene + diphosphate
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is geranylgeranyl-diphosphate diphosphate-lyase (cyclizing, taxa-4,11-diene-forming). Other names in common use include geranylgeranyl-diphosphate diphosphate-lyase (cyclizing, and taxadiene-forming). This enzyme participates in diterpenoid biosynthesis.
References
- Croteau R . 1995 . Cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene is the committed step of taxol biosynthesis in Pacific yew . J. Biol. Chem. . 270 . 8686 - 90 . 7721772 . 10.1074/jbc.270.15.8686 . Hezari . M . Zajicek . J . Vogel . BS . Lafever . RE . Lewis . NG . Croteau . R . 15 . free .
- Hezari M, Lewis NG, Croteau R . 1995 . Purification and characterization of taxa-4(5),11(12)-diene synthase from Pacific yew (Taxus brevifolia) that catalyzes the first committed step of taxol biosynthesis . Arch. Biochem. Biophys. . 322 . 437 - 44 . 7574719 . 10.1006/abbi.1995.1486 . 2 .
- Lin X, Hezari M, Koepp AE, Floss HG, Croteau R . 1996 . Mechanism of taxadiene synthase, a diterpene cyclase that catalyzes the first step of taxol biosynthesis in Pacific yew . Biochemistry . 35 . 2968 - 77 . 8608134 . 10.1021/bi9526239 . 9 .
- Hezari M, Ketchum RE, Gibson DM, Croteau R . 1997 . Taxol production and taxadiene synthase activity in Taxus canadensis cell suspension cultures . Arch. Biochem. Biophys. . 337 . 185 - 90 . 9016812 . 10.1006/abbi.1996.9772 . 2 .
- Coates RM, Williams RM, Croteau R . 2000 . Intramolecular proton transfer in the cyclization of geranylgeranyl diphosphate to the taxadiene precursor of taxol catalyzed by recombinant taxadiene synthase . Chem. Biol. . 7 . 969 - 77 . 11137819 . 10.1016/S1074-5521(00)00046-6 . 12 . free .
