TRNA guanosine-2'-O-methyltransferase explained

tRNA (guanosine-2'-O-)-methyltransferase
Ec Number:	2.1.1.34
Cas Number:	37257-01-5
Go Code:	0009020

In enzymology, a tRNA guanosine-2'-O-methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanosine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing 2'-O-methylguanosine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA guanosine-2'-O-methyltransferase. Other names in common use include transfer ribonucleate guanosine 2'-methyltransferase, tRNA guanosine 2'-methyltransferase, tRNA (guanosine 2')-methyltransferase, tRNA (Gm18) 2'-O-methyltransferase, tRNA (Gm18) methyltransferase, tRNA (guanosine-2'-O-)-methyltransferase, and S-adenosyl-L-methionine:tRNA (guanosine-2'-O-)-methyltransferase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .

References

• Gefter ML . 1969 . The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli . Biochem. Biophys. Res. Commun. . 36 . 435 - 41 . 4898378 . 10.1016/0006-291X(69)90583-X . 3 .
• Kumagai I, Watanabe K, Oshima T . 1980 . Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27 . Proc. Natl. Acad. Sci. U.S.A. . 77 . 1922 - 6 . 6990416 . 10.1073/pnas.77.4.1922 . 4 . 348621 . 1980PNAS...77.1922K . free .
• Kumagai I, Watanabe K . 1998 . Substrate recognition of tRNA (Guanosine-2'-)-methyltransferase from Thermus thermophilus HB27 . J. Biol. Chem. . 273 . 25721 - 7 . 9748240 . 10.1074/jbc.273.40.25721 . 40 . free .