TRNA (pseudouridine54-N1)-methyltransferase explained

TRNA (pseudouridine^54-N^1)-methyltransferase
Ec Number:	2.1.1.257

TRNA (pseudouridine⁵⁴-N¹)-methyltransferase (TrmY, m1Psi methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (pseudouridine54-N1)-methyltransferase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine⁵⁴ in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + N¹-methylpseudouridine⁵⁴ in tRNA

This archaeal enzyme is specific for the 54 position.

Notes and References

1. Chen HY, Yuan YA . Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase . Journal of Molecular Cell Biology . 2 . 6 . 366–74 . December 2010 . 21098051 . 10.1093/jmcb/mjq034 . free .
2. Wurm JP, Griese M, Bahr U, Held M, Heckel A, Karas M, Soppa J, Wöhnert J . Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs . RNA . 18 . 3 . 412–20 . March 2012 . 22274954 . 3285930 . 10.1261/rna.028498.111 .
3. Chatterjee K, Blaby IK, Thiaville PC, Majumder M, Grosjean H, Yuan YA, Gupta R, de Crécy-Lagard V . The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA . RNA . 18 . 3 . 421–33 . March 2012 . 22274953 . 3285931 . 10.1261/rna.030841.111 .