TRNA (guanine9-N1)-methyltransferase explained

TRNA (guanine^9-N^1)-methyltransferase
Ec Number:	2.1.1.221

TRNA (guanine⁹-N¹)-methyltransferase (Trm10p, tRNA(m1G9/m1A9)-methyltransferase, tRNA(m1G9/m1A9)MTase, tRNA (guanine-N(1)-)-methyltransferase, tRNA m1G9-methyltransferase, tRNA m1G9 MTase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine⁹-N¹)-methyltransferase.^{[1] [2]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + guanine⁹ in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + N¹-methylguanine⁹ in tRNA

The enzyme from Saccharomyces cerevisiae specifically methylates guanine⁹.

Notes and References

1. Kempenaers M, Roovers M, Oudjama Y, Tkaczuk KL, Bujnicki JM, Droogmans L . New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA . Nucleic Acids Research . 38 . 19 . 6533–43 . October 2010 . 20525789 . 2965216 . 10.1093/nar/gkq451 .
2. Jackman JE, Montange RK, Malik HS, Phizicky EM . Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9 . RNA . 9 . 5 . 574–85 . May 2003 . 12702816 . 1370423 . 10.1261/rna.5070303 .