TRNA (guanine37-N1)-methyltransferase explained

tRNA (guanine^37-N^1)-methyltransferase
Ec Number:	2.1.1.228

tRNA (guanine³⁷-N¹)-methyltransferase (TrmD, tRNA (m1G37) methyltransferase, transfer RNA (m1G37) methyltransferase, Trm5p, TRMT5, tRNA-(N1G37) methyltransferase, MJ0883 (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine³⁷-N¹)-methyltransferase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + guanine³⁷ in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + N¹-methylguanine³⁷ in tRNA

This enzyme is important for the maintenance of the correct reading frame during translation.

Notes and References

1. Takeda H, Toyooka T, Ikeuchi Y, Yokobori S, Okadome K, Takano F, Oshima T, Suzuki T, Endo Y, Hori H . The substrate specificity of tRNA (m¹G³⁷) methyltransferase (TrmD) from Aquifex aeolicus . Genes to Cells . 11 . 12 . 1353–65 . December 2006 . 17121543 . 10.1111/j.1365-2443.2006.01022.x .
2. Lee C, Kramer G, Graham DE, Appling DR . Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase . The Journal of Biological Chemistry . 282 . 38 . 27744–53 . September 2007 . 17652090 . 10.1074/jbc.m704572200 . free .
3. O'Dwyer K, Watts JM, Biswas S, Ambrad J, Barber M, Brulé H, Petit C, Holmes DJ, Zalacain M, Holmes WM . Characterization of Streptococcus pneumoniae TrmD, a tRNA methyltransferase essential for growth . Journal of Bacteriology . 186 . 8 . 2346–54 . April 2004 . 15060037 . 412112 . 10.1128/jb.186.8.2346-2354.2004 .
4. Brulé H, Elliott M, Redlak M, Zehner ZE, Holmes WM . Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein . Biochemistry . 43 . 28 . 9243–55 . July 2004 . 15248782 . 10.1021/bi049671q .
5. Goto-Ito S, Ito T, Ishii R, Muto Y, Bessho Y, Yokoyama S . Crystal structure of archaeal tRNA(m(1)G37)methyltransferase aTrm5 . Proteins . 72 . 4 . 1274–89 . September 2008 . 18384044 . 10.1002/prot.22019 .
6. Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK . Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition . The EMBO Journal . 22 . 11 . 2593–603 . June 2003 . 12773376 . 156765 . 10.1093/emboj/cdg269 .