TRNA (guanine26-N2)-dimethyltransferase explained

TRNA (guanine^26-N^2)-dimethyltransferase
Ec Number:	2.1.1.216

TRNA (guanine²⁶-N²)-dimethyltransferase (Trm1p, TRM1, tRNA (m22G26)dimethyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine²⁶-N²)-dimethyltransferase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + guanine²⁶ in tRNA

\rightleftharpoons 2 S-adenosyl-L-homocysteine + N²-dimethylguanine26 in tRNA

The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions.

Notes and References

1. Constantinesco F, Motorin Y, Grosjean H . Characterisation and enzymatic properties of tRNA(guanine 26, N (2), N (2))-dimethyltransferase (Trm1p) from Pyrococcus furiosus . Journal of Molecular Biology . 291 . 2 . 375–92 . August 1999 . 10438627 . 10.1006/jmbi.1999.2976 .
2. Constantinesco F, Benachenhou N, Motorin Y, Grosjean H . The tRNA(guanine-26,N2-N2) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli . Nucleic Acids Research . 26 . 16 . 3753–61 . August 1998 . 9685492 . 147764 . 10.1093/nar/26.16.3753 .
3. Liu J, Liu J, Stråby KB . Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m22G26)dimethyltransferase . Nucleic Acids Research . 26 . 22 . 5102–8 . November 1998 . 9801306 . 147968 . 10.1093/nar/26.22.5102 .
4. Liu J, Zhou GQ, Stråby KB . Caenorhabditis elegans ZC376.5 encodes a tRNA (m2/2G(26))dimethyltransferance in which (246)arginine is important for the enzyme activity . Gene . 226 . 1 . 73–81 . January 1999 . 10048958 . 10.1016/s0378-1119(98)00550-2 .