TRNA (guanine26-N2/guanine27-N2)-dimethyltransferase explained

TRNA (guanine^26-N^2/guanine27-N^2)-dimethyltransferase
Ec Number:	2.1.1.215

TRNA (guanine²⁶-N²/guanine²⁷-N²)-dimethyltransferase (Trm1, tRNA (N²,N²-guanine)-dimethyltransferase, tRNA (m2(2G26) methyltransferase, Trm1[tRNA (m2(2)G26) methyltransferase]) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine²⁶-N²/guanine²⁷-N²)-dimethyltransferase.^[1] This enzyme catalyses the following chemical reaction

4 S-adenosyl-L-methionine + guanine²⁶/guanine²⁷ in tRNA

\rightleftharpoons 4 S-adenosyl-L-homocysteine + N²-dimethylguanine²⁶/N²-dimethylguanine²⁷ in tRNA

The enzyme from Aquifex aeolicus is similar to the TRM1 methyltransferases of archaea and eukarya.

Notes and References

1. Awai T, Kimura S, Tomikawa C, Ochi A, Bessho Y, Yokoyama S, Ohno S, Nishikawa K, Yokogawa T, Suzuki T, Hori H . Aquifex aeolicus tRNA (N2,N2-guanine)-dimethyltransferase (Trm1) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA . The Journal of Biological Chemistry . 284 . 31 . 20467–78 . July 2009 . 19491098 . 2742811 . 10.1074/jbc.m109.020024 . free .