TRNA (guanine10-N2)-dimethyltransferase explained

TRNA (guanine^10-N^2)-dimethyltransferase
Ec Number:	2.1.1.213

TRNA (guanine¹⁰-N²)-dimethyltransferase (PAB1283, N(2),N(2)-dimethylguanosine tRNA methyltransferase, Trm-G10, PabTrm-G10, PabTrm-m2 2G10 enzyme) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine¹⁰-N²)-dimethyltransferase.^[1] This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + guanine¹⁰ in tRNA

\rightleftharpoons 2 S-adenosyl-L-homocysteine + N²-dimethylguanine¹⁰ in tRNA (overall reaction)

(1a) S-adenosyl-L-methionine + guanine¹⁰ in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + N²-methylguanine¹⁰ in tRNA

(1b) S-adenosyl-L-methionine + N²-methylguanine¹⁰ in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + N²-dimethylguanine¹⁰ in tRNA

Notes and References

1. Armengaud J, Urbonavicius J, Fernandez B, Chaussinand G, Bujnicki JM, Grosjean H . N2-methylation of guanosine at position 10 in tRNA is catalyzed by a THUMP domain-containing, S-adenosylmethionine-dependent methyltransferase, conserved in Archaea and Eukaryota . The Journal of Biological Chemistry . 279 . 35 . 37142–52 . August 2004 . 15210688 . 10.1074/jbc.m403845200 . free .