TRNA (cytosine-5-)-methyltransferase explained

tRNA (cytosine-5-)-methyltransferase
Ec Number:	2.1.1.29
Cas Number:	37256-97-6
Go Code:	0016428

In enzymology, a tRNA (cytosine-5-)-methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing 5-methylcytosine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (cytosine-5-)-methyltransferase. Other names in common use include transfer ribonucleate cytosine 5-methyltransferase, and transfer RNA cytosine 5-methyltransferase.

References

• Hurwitz J, Gold M, Anders M . 1964 . The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes . J. Biol. Chem. . 239 . 3462 - 3473 . 14245404 .
• Bjork GR, Svensson I . 1969 . Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae . Eur. J. Biochem. . 9 . 207 - 215 . 4896260 . 10.1111/j.1432-1033.1969.tb00596.x . 2 . free .
• Keith JM, Winters EM, Moss B . 1980 . Purification and characterization of a HeLa cell transfer RNA(cytosine-5-)-methyltransferase . J. Biol. Chem. . 255 . 4636 - 4644 . 7372600 . 10 .