TRNA (cytidine32/uridine32-2'-O)-methyltransferase explained

TRNA (cytidine^32/uridine^32-2'-O)-methyltransferase
Ec Number:	2.1.1.200

TRNA (cytidine³²/uridine³²-2'-O)-methyltransferase (YfhQ, tRNA:Cm³²/Um³² methyltransferase, TrMet(Xm³²), TrmJ) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytidine³²/uridine³²-2'-O)-methyltransferase.^[1] This enzyme catalyses the following chemical reaction

(1) S-adenosyl-L-methionine + cytidine³² in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methylcytidine³² in tRNA

(2) S-adenosyl-L-methionine + uridine³² in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA

In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine32 in tRNA.

Notes and References

1. Purta E, van Vliet F, Tkaczuk KL, Dunin-Horkawicz S, Mori H, Droogmans L, Bujnicki JM . The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase . BMC Molecular Biology . 7 . 23 . July 2006 . 16848900 . 1569432 . 10.1186/1471-2199-7-23 . free .