TRNA (adenine58-N1)-methyltransferase explained

TRNA (adenine^58-N^1)-methyltransferase
Ec Number:	2.1.1.220

TRNA (adenine⁵⁸-N¹)-methyltransferase (tRNA m1A58 methyltransferase, tRNA (m1A58) methyltransferase, TrmI, tRNA (m1A58) Mtase, Rv2118cp, Gcd10p-Gcd14p, Trm61p-Trm6p) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (adenine⁵⁸-N¹)-methyltransferase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + adenine⁵⁸ in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + N¹-methyladenine⁵⁸ in tRNA

The enzyme specifically methylates adenine⁵⁸ in tRNA.

Notes and References

1. Droogmans L, Roovers M, Bujnicki JM, Tricot C, Hartsch T, Stalon V, Grosjean H . Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures . Nucleic Acids Research . 31 . 8 . 2148–56 . April 2003 . 12682365 . 153742 . 10.1093/nar/gkg314 .
2. Varshney U, Ramesh V, Madabushi A, Gaur R, Subramanya HS, RajBhandary UL . Mycobacterium tuberculosis Rv2118c codes for a single-component homotetrameric m1A58 tRNA methyltransferase . Nucleic Acids Research . 32 . 3 . 1018–27 . 2004 . 14960715 . 373377 . 10.1093/nar/gkh207 .
3. Anderson J, Phan L, Hinnebusch AG . The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae . Proceedings of the National Academy of Sciences of the United States of America . 97 . 10 . 5173–8 . May 2000 . 10779558 . 25801 . 10.1073/pnas.090102597 . free .