TRNA-dihydrouridine20 synthase (NAD(P)+) explained

TRNA-dihydrouridine20 synthase (NAD(P)^+)
Ec Number:	1.3.1.91

TRNA-dihydrouridine20 synthase (NAD(P)⁺) (Dus2p, tRNA-dihydrouridine synthase 2) is an enzyme with systematic name tRNA-5,6-dihydrouracil20:NAD(P)⁺ oxidoreductase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

5,6-dihydrouracil20 in tRNA + NAD(P)⁺

\rightleftharpoons uracil20 in tRNA + NAD(P)H + H⁺

This enzyme specifically modifies uracil20 in tRNA.

Notes and References

1. Xing F, Hiley SL, Hughes TR, Phizicky EM . The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs . The Journal of Biological Chemistry . 279 . 17 . 17850–60 . April 2004 . 14970222 . 10.1074/jbc.M401221200 . free .
2. Xing F, Martzen MR, Phizicky EM . A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA . RNA . 8 . 3 . 370–81 . March 2002 . 12003496 . 1370258 . 10.1017/S1355838202029825 .
3. Rider LW, Ottosen MB, Gattis SG, Palfey BA . Mechanism of dihydrouridine synthase 2 from yeast and the importance of modifications for efficient tRNA reduction . The Journal of Biological Chemistry . 284 . 16 . 10324–33 . April 2009 . 19139092 . 2667719 . 10.1074/jbc.M806137200 . free .
4. Kato T, Daigo Y, Hayama S, Ishikawa N, Yamabuki T, Ito T, Miyamoto M, Kondo S, Nakamura Y . A novel human tRNA-dihydrouridine synthase involved in pulmonary carcinogenesis . Cancer Research . 65 . 13 . 5638–46 . July 2005 . 15994936 . 10.1158/0008-5472.CAN-05-0600 . free .