TRNA-dihydrouridine16/17 synthase (NAD(P)+) explained

TRNA-dihydrouridine16/17 synthase (NAD(P)^+)
Ec Number:	1.3.1.88

TRNA-dihydrouridine16/17 synthase (NAD(P)⁺) (Dus1p, tRNA-dihydrouridine synthase 1) is an enzyme with systematic name tRNA-5,6-dihydrouracil16/17:NAD(P)⁺ oxidoreductase.^{[1] [2]} This enzyme catalyses the following chemical reaction

(1) 5,6-dihydrouracil16 in tRNA + NAD(P)⁺

\rightleftharpoons uracil16 in tRNA + NAD(P)H + H₊

(2) 5,6-dihydrouracil17 in tRNA + NAD(P)⁺

\rightleftharpoons uracil17 in tRNA + NAD(P)H + H⁺

This enzyme specifically modifies uracil16 and uracil17 in tRNA.

Notes and References

1. Xing F, Hiley SL, Hughes TR, Phizicky EM . The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs . The Journal of Biological Chemistry . 279 . 17 . 17850–60 . April 2004 . 14970222 . 10.1074/jbc.M401221200 . free .
2. Xing F, Martzen MR, Phizicky EM . A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA . RNA . 8 . 3 . 370–81 . March 2002 . 12003496 . 1370258 . 10.1017/S1355838202029825 .