TRNA:m4X modification enzyme explained

TRNA:m4X modification enzyme
Ec Number:	2.1.1.225

TRNA:m4X modification enzyme (TRM13, Trm13p, tRNA:Xm4 modification enzyme) is an enzyme with systematic name S-adenosyl-L-methionine:tRNAPro/His/Gly(GCC) (cytidine/adenosine4-2'-O)-methyltransferase.^[1] This enzyme catalyses the following chemical reaction

(1) S-adenosyl-L-methionine + cytidine⁴ in tRNAPro

\rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methylcytidine⁴ in tRNAPro

(2) S-adenosyl-L-methionine + cytidine⁴ in tRNAGly(GCC)

\rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methylcytidine⁴ in tRNAGly(GCC)

(3) S-adenosyl-L-methionine + adenosine⁴ in tRNAHis

\rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methyladenosine⁴ in tRNAHis

The enzyme from Saccharomyces cerevisiae 2'-O-methylates cytidine⁴ in tRNAPro and tRNAGly(GCC), and adenosine⁴ in tRNAHis.

Notes and References

1. Wilkinson ML, Crary SM, Jackman JE, Grayhack EJ, Phizicky EM . The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4 . RNA . 13 . 3 . 404–13 . March 2007 . 17242307 . 1800514 . 10.1261/rna.399607 .