Sulfur carrier protein ThiS adenylyltransferase explained

Sulfur carrier protein ThiS adenylyltransferase
Ec Number:	2.7.7.73

Sulfur carrier protein ThiS adenylyltransferase (thiF (gene)) is an enzyme with systematic name ATP:(ThiS) adenylyltransferase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

ATP + [ThiS]

\rightleftharpoons diphosphate + adenylyl-[ThiS]

This enzyme binds Zn²⁺. The enzyme catalyses the adenylation of ThiS, a sulfur carrier protein involved in the biosynthesis of thiamine.

Notes and References

1. Taylor SV, Kelleher NL, Kinsland C, Chiu HJ, Costello CA, Backstrom AD, McLafferty FW, Begley TP . Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation . The Journal of Biological Chemistry . 273 . 26 . 16555–60 . June 1998 . 9632726 . 10.1074/jbc.273.26.16555 . free .
2. Xi J, Ge Y, Kinsland C, McLafferty FW, Begley TP . Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex . Proceedings of the National Academy of Sciences of the United States of America . 98 . 15 . 8513–8 . July 2001 . 11438688 . 37467 . 10.1073/pnas.141226698 . free .
3. Duda DM, Walden H, Sfondouris J, Schulman BA . Structural analysis of Escherichia coli ThiF . Journal of Molecular Biology . 349 . 4 . 774–86 . June 2005 . 15896804 . 10.1016/j.jmb.2005.04.011 .
4. Lehmann C, Begley TP, Ealick SE . Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis . Biochemistry . 45 . 1 . 11–9 . January 2006 . 16388576 . 2566941 . 10.1021/bi051502y .