Sucrose α-glucosidase explained

Sucrose α-glucosidase
Ec Number:	3.2.1.48
Cas Number:	37288-39-4

Sucrose α-glucosidase (sucrose α-glucohydrolase, sucrase, sucrase-isomaltase, sucrose.α.-glucohydrolase, intestinal sucrase, sucrase(invertase)) is an enzyme with systematic name sucrose-α-D-glucohydrolase.^{[1] [2] [3] [4] [5] [6]} It catalyses the hydrolysis of sucrose and maltose by an α-D-glucosidase-type action.

This enzyme is isolated from intestinal mucosa as a single polypeptide chain. The human sucrase-isomaltase is a dual-function enzyme with two GH31 domains, one serving as the isomaltase, the other serving as a sucrose α-glucosidase.

Notes and References

1. Conklin KA, Yamashiro KM, Gray GM . Human intestinal sucrase-isomaltase. Identification of free sucrase and isomaltase and cleavage of the hybrid into active distinct subunits . The Journal of Biological Chemistry . 250 . 15 . 5735–41 . August 1975 . 10.1016/S0021-9258(19)41116-2 . 807575 . free .
2. Hauri HP, Quaroni A, Isselbacher KJ . Biogenesis of intestinal plasma membrane: posttranslational route and cleavage of sucrase-isomaltase . Proceedings of the National Academy of Sciences of the United States of America . 76 . 10 . 5183–6 . October 1979 . 291933 . 413104 . 10.1073/pnas.76.10.5183 . free .
3. Kolínská J, Kraml J . Separation and characterization of sucrose-isomaltase and of glucoamylase of rat intestine . Biochimica et Biophysica Acta (BBA) - Enzymology . 284 . 1 . 235–47 . September 1972 . 5073761 . 10.1016/0005-2744(72)90062-9 .
4. Sigrist H, Ronner P, Semenza G . A hydrophobic form of the small-intestinal sucrase-isomaltase complex . Biochimica et Biophysica Acta (BBA) - Biomembranes . 406 . 3 . 433–46 . October 1975 . 1182172 . 10.1016/0005-2736(75)90022-x .
5. Sjöström H, Norén O, Christiansen L, Wacker H, Semenza G . A fully active, two-active-site, single-chain sucrase.isomaltase from pig small intestine. Implications for the biosynthesis of a mammalian integral stalked membrane protein . The Journal of Biological Chemistry . 255 . 23 . 11332–8 . December 1980 . 10.1016/S0021-9258(19)70296-8 . 7002920 . free .
6. Takesue Y . Purification and properties of rabbit intestinal sucrase . Journal of Biochemistry . 65 . 4 . 545–52 . April 1969 . 10.1093/oxfordjournals.jbchem.a129048 . 5804876 .