Sterol 24-C-methyltransferase explained
| sterol 24-C-methyltransferase |
| Ec Number: | 2.1.1.41 |
| Cas Number: | 37257-07-1 |
| Go Code: | 0003838 |
In enzymology, a sterol 24-C-methyltransferase is an enzyme that catalyzes the chemical reaction
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol
S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol
Thus, the two substrates of this enzyme are S-adenosyl methionine and 5alpha-cholesta-8,24-dien-3beta-ol, whereas its two products are S-adenosylhomocysteine and 24-methylene-5alpha-cholest-8-en-3beta-ol.
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:zymosterol 24-C-methyltransferase. Other names in common use include Delta24-methyltransferase, Delta24-sterol methyltransferase, zymosterol-24-methyltransferase, S-adenosyl-4-methionine:sterol Delta24-methyltransferase, SMT1, 24-sterol C-methyltransferase, S-adenosyl-L-methionine:Delta24(23)-sterol methyltransferase, and phytosterol methyltransferase. This enzyme participates in biosynthesis of steroids. It employs one cofactor, glutathione.
References
- Moore JT, Gaylor JL . 1969 . Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast . J. Biol. Chem. . 244 . 6334 - 40 . 5354959 . 23 .
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- MD, Zhou W, Lopez M, Nes WD . 1997 ., Stereochemical features of C-methylation on the path to Delta24(28)-methylene and Delta24(28)-ethylidene sterols: studies on the recombinant phytosterol methyl transferase from Arabidopsis thaliana . Tetrahedron Lett. . 38 . 6115 - 6118 . 10.1016/S0040-4039(97)01386-5 . 35 .
- Bouvier-Nave P, Husselstein T, Benveniste P . 1998 . Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis . Eur. J. Biochem. . 256 . 88 - 96 . 9746350 . 10.1046/j.1432-1327.1998.2560088.x . 1 .
- Nes WD, McCourt BS, Zhou WX, Ma J, Marshall JA, Peek LA, Brennan M . 1998 . Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: delta 24(25)- to delta 24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae . Arch. Biochem. Biophys. . 353 . 297 - 311 . 9606964 . 10.1006/abbi.1998.0665 . 2 .
