Steroid 15beta-monooxygenase explained
| Steroid 15beta-monooxygenase |
| Ec Number: | 1.14.15.8 |
Steroid 15beta-monooxygenase (cytochrome P-450meg, cytochrome P450meg, steroid 15beta-hydroxylase, CYP106A2, BmCYP106A2) is an enzyme with systematic name progesterone,reduced-ferredoxin:oxygen oxidoreductase (15beta-hydroxylating) .[1] [2] [3] [4] [5] This enzyme catalyses the following chemical reaction
progesterone + reduced ferredoxin + O2
15beta-hydroxyprogesterone + oxidized
ferredoxin + H
2O
The enzyme from Bacillus megaterium hydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta.
Notes and References
- Berg A, Ingelman-Sundberg M, Gustafsson JA . Purification and characterization of cytochrome P-450meg . The Journal of Biological Chemistry . 254 . 12 . 5264–71 . June 1979 . 10.1016/S0021-9258(18)50589-5 . 109432 . free .
- Berg A, Gustafsson JA, Ingelman-Sundberg M . Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium . The Journal of Biological Chemistry . 251 . 9 . 2831–8 . May 1976 . 10.1016/S0021-9258(17)33564-0 . 177422 . free .
- Lisurek M, Kang MJ, Hartmann RW, Bernhardt R . Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry . Biochemical and Biophysical Research Communications . 319 . 2 . 677–82 . June 2004 . 15178459 . 10.1016/j.bbrc.2004.05.037 .
- Goñi G, Zöllner A, Lisurek M, Velázquez-Campoy A, Pinto S, Gómez-Moreno C, Hannemann F, Bernhardt R, Medina M . Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368 . Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics . 1794 . 11 . 1635–42 . November 2009 . 19635596 . 10.1016/j.bbapap.2009.07.012 .
- Lisurek M, Simgen B, Antes I, Bernhardt R . Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation . ChemBioChem . 9 . 9 . 1439–49 . June 2008 . 18481342 . 10.1002/cbic.200700670 . 10667895 .
