Soluble quinoprotein glucose dehydrogenase explained

Soluble quinoprotein glucose dehydrogenase
Ec Number:	1.1.99.35

Soluble quinoprotein glucose dehydrogenase (soluble glucose dehydrogenase, sGDH, glucose dehydrogenase (PQQ-dependent)) is an enzyme with systematic name D-glucose:acceptor oxidoreductase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

D-glucose + acceptor

\rightleftharpoons D-glucono-1,5-lactone + reduced acceptor

This soluble periplasmic enzyme contains PQQ as prosthetic group, and is bound to a calcium ion. Electron acceptor is not known.

Notes and References

1. Crystalline quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus . Geiger O, Gorisch H . Biochemistry . 1986 . 25 . 6043–6048 . 10.1021/bi00368a031.
2. Dokter P, Frank J, Duine JA . Purification and characterization of quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus L.M.D. 79.41 . The Biochemical Journal . 239 . 1 . 163–7 . October 1986 . 3800975 . 1147254 .
3. Cleton-Jansen AM, Goosen N, Wenzel TJ, van de Putte P . Cloning of the gene encoding quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus: evidence for the presence of a second enzyme . Journal of Bacteriology . 170 . 5 . 2121–5 . May 1988 . 2834325 . 211095 .
4. Matsushita K, Shinagawa E, Adachi O, Ameyama M . Quinoprotein D-glucose dehydrogenase of the Acinetobacter calcoaceticus respiratory chain: membrane-bound and soluble forms are different molecular species . Biochemistry . 28 . 15 . 6276–80 . July 1989 . 2551369 . 10.1021/bi00441a020 .
5. Oubrie A, Dijkstra BW . Structural requirements of pyrroloquinoline quinone dependent enzymatic reactions . Protein Science . 9 . 7 . 1265–73 . July 2000 . 10933491 . 2144678 . 10.1110/ps.9.7.1265 .
6. Soluble and membrane-bound quinoprotein D-glucose dehydrogenases of the Acinetobacter calcoaceticus : the binding process of PQQ to the apoenzymes . Matsushita, K. . Toyama, H. . Ameyama, M. . Adachi, O. . Dewanti, A. . Duine, J.A. . Biosci. Biotechnol. Biochem. . 1995 . 59 . 1548–1555 . 10.1271/bbb.59.1548.