Site-specific DNA-methyltransferase (cytosine-N4-specific) explained

Site-specific DNA-methyltransferase (cytosine-N4-specific)
Ec Number:	2.1.1.113
Cas Number:	169592-50-1

Site-specific DNA-methyltransferase (cytosine-N4-specific) (modification methylase, restriction-modification system, DNA[cytosine-N4]methyltransferase, m4C-forming MTase, S-adenosyl-L-methionine:DNA-cytosine 4-N-methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:DNA-cytosine N4-methyltransferase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction:

S-adenosyl-L-methionine + DNA cytosine

\rightleftharpoons S-adenosyl-L-homocysteine + DNA N4-methylcytosine

This is a large group of enzymes.^[5]

See also

• DNA methyltransferase

Notes and References

1. Kessler C, Manta V . Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3) . Gene . 92 . 1-2 . 1–248 . August 1990 . 2172084 . 10.1016/0378-1119(90)90486-B .
2. Klimasauskas S, Timinskas A, Menkevicius S, Butkienè D, Butkus V, Janulaitis A . Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases . Nucleic Acids Research . 17 . 23 . 9823–32 . December 1989 . 2690010 . 335216 . 10.1093/nar/17.23.9823 .
3. Roberts RJ . Restriction enzymes and their isoschizomers . Nucleic Acids Research . 18 Suppl . 2331–65 . April 1990 . 2159140 . 331877 . 10.1093/nar/18.suppl.2331 .
4. Yuan R . Structure and mechanism of multifunctional restriction endonucleases . Annual Review of Biochemistry . 50 . 285–319 . 1981 . 6267988 . 10.1146/annurev.bi.50.070181.001441 .
5. Web site: A complete listing of all of these enzymes . Roberts RJ . The Restriction Enzyme Database .