Svedberg Explained

In chemistry, a Svedberg unit or svedberg (symbol S, sometimes Sv) is a non-SI metric unit for sedimentation coefficients. The Svedberg unit offers a measure of a particle's size indirectly based on its sedimentation rate under acceleration (i.e. how fast a particle of given size and shape settles out of suspension). The svedberg is a measure of time, defined as exactly 10−13 seconds (100 fs).

For biological macromolecules and cell organelles like ribosomes, the sedimentation rate is typically measured as the rate of travel in a centrifuge tube subjected to high g-force.

The svedberg (S) is distinct from the SI unit sievert or the non-SI unit sverdrup, which also use the symbol Sv, and to the SI unit Siemens which uses the symbol S too.

Naming

The unit is named after the Swedish chemist Theodor Svedberg (1884–1971), winner of the 1926 Nobel Prize[1] in chemistry for his work on disperse systems, colloids and his invention of the ultracentrifuge.[2]

Factors

The Svedberg coefficient is a nonlinear function. A particle's mass, density, and shape will determine its S value. The S value depends on the frictional forces retarding its movement, which, in turn, are related to the average cross-sectional area of the particle.[3]

The sedimentation coefficient is the ratio of the speed of a substance in a centrifuge to its acceleration in comparable units. A substance with a sedimentation coefficient of 26S will travel at 26 micrometers per second under the influence of an acceleration of a million gravities (107 m/s2). Centrifugal acceleration is given as ; where r is the radial distance from the rotation axis and ω is the angular velocity in radians per second.

Bigger particles tend to sediment faster and so have higher Svedberg values.

Svedberg units are not directly additive since they represent a rate of sedimentation, not weight.[3]

Use

In centrifugation of small biochemical species, a convention has developed in which sedimentation coefficients are expressed in the Svedberg units.

See also

External links

Notes and References

  1. Web site: The Nobel Prize in Chemistry 1926.
  2. Web site: Koehler . Christopher S. W. . 2003 . Developing the Ultracentrifuge . https://web.archive.org/web/20231002182851/https://pubsapp.acs.org/subscribe/archive/tcaw/12/i02/pdf/203chronicles.pdf . 2 October 2023 .
  3. Book: Slonczewski . Joan . John Watkins . Foster . Microbiology: An Evolving Science . New York . W.W. Norton . 2009 . 9780393978575 .