| Cabinet Name: | Second Cabinet of Kostas Karamanlis |
| Jurisdiction: | Greece |
| Flag: | Flag of Greece.svg |
| Government Head: | Kostas Karamanlis |
| State Head: | Karolos Papoulias |
| Political Parties: | New Democracy (ND) |
| Opposition Parties: | Panhellenic Socialist Movement (PASOK) Communist Party of Greece (KKE) Coalition of the Radical Left (SYRIZA) Popular Orthodox Rally (LAOS) |
| Opposition Leader: | George Papandreou |
| Election: | 2007 Greek legislative election |
| Legislature Term: | 12th (2007–2009) |
| Previous: | Kostas Karamanlis I cabinet |
| Successor: | George Papandreou Cabinet |
Kostas Karamanlis served as a Prime Minister of Greece for two consecutive terms. During his incumbency, the period 2004-2009, he formed two cabinets. The second Kostas Karamanlis cabinet was formed after the 2007 elections and was succeeded by the George Papandreou Cabinet.
On September 18, 2007, after the re-election of his government, Prime Minister Kostas Karamanlis announced his second cabinet, the members of which assumed office on 19 September 2007.
As part of a reorganisation of the ministries, the incoming government announced:
On 7 January 2009, Karamanlis announced another major cabinet reshuffle.
| Office | Incumbent | Since |
|---|---|---|
| Prime Minister | Kostas Karamanlis | 10 March 2004 |
| Minister for the Interior and Public Order | Prokopis Pavlopoulos | 10 March 2004 |
| Minister for Economy and Finance | Georgios Alogoskoufis | 10 March 2004 |
| Yannis Papathanasiou | 8 January 2009 | |
| Minister for Foreign Affairs | Dora Bakoyannis | 15 February 2006 |
| Minister for National Defence | Vangelis Meimarakis | 15 February 2006 |
| Minister for Development | Christos Folias | 19 September 2007 |
| Kostis Hatzidakis | 8 January 2009 | |
| Minister for the Environment, Physical Planning and Public Works | Georgios Souflias | 10 March 2004 |
| Minister for National Education and Religious Affairs | Evripidis Stylianidis | 19 September 2007 |
| Aris Spiliotopoulos | 8 January 2009 | |
| Minister for Employment and Social Protection | Vasilios Magginas | 30 April 2007 |
| Fani Palli-Petralia | 18 December 2007 | |
| Minister for Health and Social Solidarity | Dimitris Avramopoulos | 15 February 2006 |
| Minister for Rural Development and Food | Alexandros Kontos | 19 September 2007 |
| Sotirios Hatzigakis | 8 January 2009 | |
| Minister for Justice | Sotirios Hatzigakis | 19 September 2007 |
| Nikos Dendias | 8 January 2009 | |
| Minister for Culture and Athletics | Michalis Liapis | 19 September 2007 |
| Antonis Samaras | 8 January 2009 | |
| Minister for Tourism | Aris Spiliotopoulos | 19 September 2007 |
| Kostas Markopoulos | 8 January 2009 | |
| Minister for Transport and Communications | Kostis Hatzidakis | 19 September 2007 |
| Evripidis Stylianidis | 8 January 2009 | |
| Minister for Mercantile Marine and Island Policy | Georgios Voulgarakis | 19 September 2007 |
| Anastasios Papaligouras | 12 September 2008 | |
| Minister for Macedonia–Thrace | Margaritis Tzimas | 19 September 2007 |
| Stavros Kalafatis | 8 January 2009 | |
| Minister for State | Theodoros Roussopoulos | 10 March 2004 |
| Prokopis Pavlopoulos (offices merged) | 24 October 2008 | |
| Alternate Minister of Interior and Public Order | Christos Markogiannakis | 8 January 2009 |