Rhamnogalacturonan rhamnohydrolase explained

Rhamnogalacturonan rhamnohydrolase
Ec Number:	3.2.1.174

Rhamnogalacturonan rhamnohydrolase (RG-rhamnohydrolase, RG alpha-L-rhamnopyranohydrolase) is an enzyme with systematic name rhamnogalacturonan oligosaccharide alpha-L-Rha-(1->4)-alpha-D-GalA rhamnohydrolase.^{[1] [2]} This enzyme catalyses the following chemical reaction

Exohydrolysis of the alpha-L-Rha-(1->4)-alpha-D-GalA bond in rhamnogalacturonan oligosaccharides with initial inversion of configuration releasing beta-L-rhamnose from the non-reducing end of rhamnogalacturonan oligosaccharides.

The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.

Notes and References

1. Pitson SM, Mutter M, van den Broek LA, Voragen AG, Beldman G . Stereochemical course of hydrolysis catalysed by alpha-L-rhamnosyl and alpha-D-galacturonosyl hydrolases from Aspergillus aculeatus . Biochemical and Biophysical Research Communications . 242 . 3 . 552–9 . January 1998 . 9464254 . 10.1006/bbrc.1997.8009 .
2. Mutter M, Beldman G, Schols HA, Voragen AG . Rhamnogalacturonan alpha-L-rhamnopyranohydrolase. A novel enzyme specific for the terminal nonreducing rhamnosyl unit in rhamnogalacturonan regions of pectin . Plant Physiology . 106 . 1 . 241–50 . September 1994 . 7972516 . 159522 . 10.1104/pp.106.1.241 .