Rhamnogalacturonan hydrolase explained
| Rhamnogalacturonan hydrolase |
| Ec Number: | 3.2.1.171 |
Rhamnogalacturonan hydrolase (rhamnogalacturonase A, RGase A, RG-hydrolase) is an enzyme with systematic name rhamnogalacturonan alpha-D-GalA-(1->2)-alpha-L-Rha hydrolase.[1] [2] [3] [4] [5] This enzyme catalyses the following chemical reaction
Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.
The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.
Notes and References
- Petersen TN, Kauppinen S, Larsen S . The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel beta helix . Structure . 5 . 4 . 533–44 . April 1997 . 9115442 . 10.1016/s0969-2126(97)00209-8 . free .
- Kofod LV, Kauppinen S, Christgau S, Andersen LN, Heldt-Hansen HP, Dörreich K, Dalbøge H . Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus . The Journal of Biological Chemistry . 269 . 46 . 29182–9 . November 1994 . 7961884 .
- Azadi P, O'Neill MA, Bergmann C, Darvill AG, Albersheim P . The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase . Glycobiology . 5 . 8 . 783–9 . December 1995 . 8720076 . 10.1093/glycob/5.8.783 .
- Petersen TN, Christgau S, Kofod LV, Kauppinen S, Johnson AH, Larsen S . Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus . Acta Crystallographica Section D . 53 . Pt 1 . 105–7 . January 1997 . 15299976 . 10.1107/s0907444996010785 .
- Pitson SM, Mutter M, van den Broek LA, Voragen AG, Beldman G . Stereochemical course of hydrolysis catalysed by alpha-L-rhamnosyl and alpha-D-galacturonosyl hydrolases from Aspergillus aculeatus . Biochemical and Biophysical Research Communications . 242 . 3 . 552–9 . January 1998 . 9464254 . 10.1006/bbrc.1997.8009 .
