Random hexamer explained

A random hexamer or random hexonucleotides are for various PCR applications such as rolling circle amplification to prime the DNA.

They are oligonucleotide sequences of 6 bases which are synthesised entirely randomly to give a numerous range of sequences that have the potential to anneal at many random points on a DNA sequence and act as a primer to commence first strand cDNA synthesis.^{[1] [2] [3]}

Notes and References

1. Web site: Reverse Transcription Applications - US .
2. https://www.thermoscientificbio.com/general-reagents-and-accessories/primers-for-cdna-synthesis
3. 2896536 . 20395217 . 10.1093/nar/gkq224 . 38 . Biases in Illumina transcriptome sequencing caused by random hexamer priming . 2010 . Nucleic Acids Res. . e131 . Hansen . KD . Brenner . SE . Dudoit . S . 12 . Sandrine Dudoit.