Random chimeragenesis on transient templates explained

Random chimeragenesis on transient templates (RACHITT) is a method to perform molecular mutagenesis at a high recombination rate.[1] For example, RACHITT can be used to generate increased rate and extent of biodesulfurization of diesel by modification of dibenzothiophene mono-oxygenase. DNA shuffling is a similar but less powerful method used in directed evolution experiments.

Notes and References

  1. Coco. Wayne M.. Levinson, William E. . Crist, Michael J. . Hektor, Harm J. . Darzins, Aldis . Pienkos, Philip T. . Squires, Charles H. . Monticello, Daniel J. . DNA shuffling method for generating highly recombined genes and evolved enzymes. Nature Biotechnology. 31 March 2001. 19. 4. 354–359. 10.1038/86744. 11283594. 35360374 .

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