RRNA (guanine-N2-)-methyltransferase explained

rRNA (guanine-N2-)-methyltransferase
Ec Number:	2.1.1.52
Cas Number:	50812-26-5
Go Code:	0008990

In enzymology, a rRNA (guanine-N2-)-methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + rRNA

\rightleftharpoons S-adenosyl-L-homocysteine + rRNA containing N₂-methylguanine

Thus, the two substrates of this enzyme are S-adenosyl methionine and rRNA, whereas its two products are S-adenosylhomocysteine and rRNA containing N2-methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:rRNA (guanine-N2-)-methyltransferase. Other names in common use include ribosomal ribonucleate guanine-2-methyltransferase, and S-adenosyl-L-methionine:rRNA (guanine-2-N-)-methyltransferase.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .

References

• Isaksson LA . Partial purification of ribosomal RNA(m1G)- and rRNA(m2G)-methylases from Escherichia coli and demonstration of some proteins affecting their apparent activity . Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis . 312 . 1 . 122–33 . June 1973 . 4580201 . 10.1016/0005-2787(73)90057-9 .