Pyruvate dehydrogenase (quinone) explained

Pyruvate dehydrogenase (quinone)
Ec Number:	1.2.5.1

Pyruvate dehydrogenase (quinone) (pyruvate dehydrogenase, pyruvic dehydrogenase, pyruvic (cytochrome b1) dehydrogenase, pyruvate:ubiquinone-8-oxidoreductase, pyruvate oxidase (ambiguous)) is an enzyme with systematic name pyruvate:ubiquinone oxidoreductase.^{[1] [2] [3] [4] [5] [6] [7] [8]} This enzyme catalyses the following chemical reaction

pyruvate + ubiquinone + H₂O

\rightleftharpoons acetate + CO₂ + ubiquinol

This bacterial enzyme is located on the inner surface of the cytoplasmic membrane.

Notes and References

1. Recny MA, Hager LP . Reconstitution of native Escherichia coli pyruvate oxidase from apoenzyme monomers and FAD . The Journal of Biological Chemistry . 257 . 21 . 12878–86 . November 1982 . 6752142 .
2. Cunningham CC, Hager LP . Reactivation of the lipid-depleted pyruvate oxidase system from Escherichia coli with cell envelope neutral lipids . The Journal of Biological Chemistry . 250 . 18 . 7139–46 . September 1975 . 1100621 .
3. Koland JG, Miller MJ, Gennis RB . Reconstitution of the membrane-bound, ubiquinone-dependent pyruvate oxidase respiratory chain of Escherichia coli with the cytochrome d terminal oxidase . Biochemistry . 23 . 3 . 445–53 . January 1984 . 6367818 . 10.1021/bi00298a008 .
4. Grabau C, Cronan JE . In vivo function of Escherichia coli pyruvate oxidase specifically requires a functional lipid binding site . Biochemistry . 25 . 13 . 3748–51 . July 1986 . 3527254 . 10.1021/bi00361a003 .
5. Wang AY, Chang YY, Cronan JE . Role of the tetrameric structure of Escherichia coli pyruvate oxidase in enzyme activation and lipid binding . The Journal of Biological Chemistry . 266 . 17 . 10959–66 . June 1991 . 2040613 .
6. Chang YY, Cronan JE . Sulfhydryl chemistry detects three conformations of the lipid binding region of Escherichia coli pyruvate oxidase . Biochemistry . 36 . 39 . 11564–73 . September 1997 . 9305946 . 10.1021/bi9709102 .
7. O'Brien TA, Schrock HL, Russell P, Blake R, Gennis RB . Preparation of Escherichia coli pyruvate oxidase utilizing a thiamine pyrophosphate affinity column . Biochimica et Biophysica Acta (BBA) - Enzymology . 452 . 1 . 13–29 . November 1976 . 791368 . 10.1016/0005-2744(76)90054-1 .
8. Bertagnolli BL, Hager LP . Role of flavin in acetoin production by two bacterial pyruvate oxidases . Archives of Biochemistry and Biophysics . 300 . 1 . 364–71 . January 1993 . 8424670 . 10.1006/abbi.1993.1049 .