Pyrimidine oxygenase explained

Pyrimidine oxygenase
Ec Number:	1.14.99.46

Pyrimidine oxygenase (RutA) is an enzyme with systematic name ).^{[1] [2]} This enzyme catalyses the following chemical reaction

(1) uracil + FMNH₂ + O₂

\rightleftharpoons (Z)-3-ureidoacrylate peracid + FMN

(2) thymine + FMNH₂ + O₂

\rightleftharpoons (Z)-2-methylureidoacrylate peracid + FMN

In vitro the product (Z)-3-ureidoacrylate peracid is spontaneously reduced to ureidoacrylate.

Notes and References

1. Mukherjee T, Zhang Y, Abdelwahed S, Ealick SE, Begley TP . Catalysis of a flavoenzyme-mediated amide hydrolysis . Journal of the American Chemical Society . 132 . 16 . 5550–1 . April 2010 . 20369853 . 2873085 . 10.1021/ja9107676 .
2. Kim KS, Pelton JG, Inwood WB, Andersen U, Kustu S, Wemmer DE . The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems . Journal of Bacteriology . 192 . 16 . 4089–102 . August 2010 . 20400551 . 2916427 . 10.1128/JB.00201-10 .