Pyrethroid hydrolase explained

Pyrethroid hydrolase
Ec Number:	3.1.1.88

The enzyme pyrethroid hydrolase (EC 3.1.1.88, pyrethroid-hydrolyzing carboxylesterase, pyrethroid-hydrolysing esterase, pyrethroid-hydrolyzing esterase, pyrethroid-selective esterase, pyrethroid-cleaving enzyme, permethrinase, PytH, EstP; systematic name pyrethroid-ester hydrolase)^{[1] [2] [3] [4] [5] [6]} catalyses the reaction

trans-permethrin + H₂O

\rightleftharpoons (3-phenoxyphenyl)methanol + (1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate

The enzyme is involved in degradation of pyrethroid pesticides.

Notes and References

1. Wang BZ, Guo P, Hang BJ, Li L, He J, Li SP . Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product . Applied and Environmental Microbiology . 75 . 17 . 5496–500 . September 2009 . 19581484 . 2737939 . 10.1128/aem.01298-09 .
2. Wu PC, Liu YH, Wang ZY, Zhang XY, Li H, Liang WQ, Luo N, Hu JM, Lu JQ, Luan TG, Cao LX . Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from Klebsiella sp. strain ZD112 . Journal of Agricultural and Food Chemistry . 54 . 3 . 836–42 . February 2006 . 16448191 . 10.1021/jf052691u .
3. Liang WQ, Wang ZY, Li H, Wu PC, Hu JM, Luo N, Cao LX, Liu YH . Purification and characterization of a novel pyrethroid hydrolase from Aspergillus niger ZD11 . Journal of Agricultural and Food Chemistry . 53 . 19 . 7415–20 . September 2005 . 16159167 . 10.1021/jf051460k .
4. Stok JE, Huang H, Jones PD, Wheelock CE, Morisseau C, Hammock BD . Identification, expression, and purification of a pyrethroid-hydrolyzing carboxylesterase from mouse liver microsomes . The Journal of Biological Chemistry . 279 . 28 . 29863–9 . July 2004 . 15123619 . 10.1074/jbc.m403673200 . free .
5. Maloney SE, Maule A, Smith AR . Purification and preliminary characterization of permethrinase from a pyrethroid-transforming strain of Bacillus cereus . Applied and Environmental Microbiology . 59 . 7 . 2007–13 . July 1993 . 8357241 . 182228 .
6. Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase . Guo, P. . Wang, B. . Hang, B. . Li, L. . Ali, W. . He, J. . Li, S. . Int. Biodeter. Biodegrad. . 2009 . 63 . 8 . 1107–1112 . 10.1016/j.ibiod.2009.09.008.