Pseudomonas exotoxin explained

The Pseudomonas exotoxin (or exotoxin A) is an exotoxin produced by Pseudomonas aeruginosa.^[1] Vibrio cholerae produces a similar protein called the Cholix toxin .^[2]

It inhibits elongation factor-2. It does so by ADP-ribosylation of EF2 using NAD+. This then causes the elongation of polypeptides to cease. This mechanism is similar to that of diphtheria toxin.^[3]

It has been investigated as a treatment for hepatitis B^[4] and cancer.^[5]

External links

Yates SP, Taylor PL, Jørgensen R, Ferraris D, Zhang J, Andersen GR, Merrill AR . Structure-function analysis of water-soluble inhibitors of the catalytic domain of exotoxin A from Pseudomonas aeruginosa . The Biochemical Journal . 385 . Pt 3 . 667–75 . February 2005 . 15458385 . 1134741 . 10.1042/BJ20041480 .
Jørgensen R, Purdy AE, Fieldhouse RJ, Kimber MS, Bartlett DH, Merrill AR . Cholix toxin, a novel ADP-ribosylating factor from Vibrio cholerae . The Journal of Biological Chemistry . 283 . 16 . 10671–8 . April 2008 . 18276581 . 10.1074/jbc.M710008200 . free .
Yates SP, Merrill AR . Elucidation of eukaryotic elongation factor-2 contact sites within the catalytic domain of Pseudomonas aeruginosa exotoxin A . The Biochemical Journal . 379 . Pt 3 . 563–72 . May 2004 . 14733615 . 1224111 . 10.1042/BJ20031731 .
Hafkemeyer P, Brinkmann U, Brinkmann E, Pastan I, Blum HE, Baumert TF . Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells . World Journal of Gastroenterology . 14 . 18 . 2810–7 . May 2008 . 18473403 . 2710720 . 10.3748/wjg.14.2810 . free .
Stuckey DW, Hingtgen SD, Karakas N, Rich BE, Shah K . Engineering toxin-resistant therapeutic stem cells to treat brain tumors . Stem Cells . 33 . 2 . 589–600 . February 2015 . 25346520 . 4305025 . 10.1002/stem.1874 .