Pseudaminic acid synthase explained

Pseudaminic acid synthase
Ec Number:	2.5.1.97

Pseudaminic acid synthase (PseI, NeuB3) is an enzyme with systematic name phosphoenolpyruvate:2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose transferase (phosphate-hydrolysing, 2,7-acetylamino-transferring, 2-carboxy-2-oxoethyl-forming).^[1] This enzyme catalyses the following chemical reaction

phosphoenolpyruvate + 2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose + H₂O

\rightleftharpoons 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid + phosphate

The enzyme requires a divalent metal ion, preferably Mn²⁺ and Co²⁺.

Notes and References

1. Chou WK, Dick S, Wakarchuk WW, Tanner ME . Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase . The Journal of Biological Chemistry . 280 . 43 . 35922–8 . October 2005 . 16120604 . 10.1074/jbc.M507483200 . free .