Hydrolyzed protein explained

Hydrolyzed protein is a solution derived from the hydrolysis of a protein into its component amino acids and peptides. While many means of achieving this exist, most common is prolonged heating with hydrochloric acid, sometimes with an enzyme such as pancreatic protease to simulate the naturally occurring hydrolytic process.

Uses

Protein hydrolysis is a useful route to the isolation of individual amino acids.^[1] Examples include cystine from hydrolysis of hair, tryptophane from casein,^[2] histidine from red blood cells,^[3] and arginine from gelatin.^[4]

Common hydrolyzed products used in food are hydrolyzed vegetable protein and yeast extract, which are used as flavor enhancers because the hydrolysis of the protein produces free glutamic acid. The non-protein components in these products also contribute to the flavor.^[5]

Some hydrolyzed beef protein powders are used for specialized diets for athletes.^[6]

Protein hydrolysis can be used to modify the allergenic properties of infant formula. Reducing the size of cow milk proteins in the formula makes it more suitable for consumption by babies suffering from milk protein intolerance. The US FDA has approved a label for this usage of partially-hydrolyzed proteins in 2017,^[7] but a meta-analysis published the same year shows insufficient evidence for this use.^[8]

Hydrolyzed protein is also used in certain specially formulated hypoallergenic pet foods, notably dog foods for dogs and puppies that suffer from allergies caused by certain protein types in standard commercial dog food brands. The protein contents of the foods are split into peptides which reduces the likelihood for an animal's immune system recognizing an allergic threat. Hydrolyzed protein diets for cats are often recommended for felines with food allergies and certain types of digestive issues.^[9]

See also

• Acceptable daily intake
• Acid-hydrolyzed vegetable protein
• E number
• Food allergy
• Food intolerance
• Food labeling regulations
• Glutamic acid
• Monosodium glutamate
• Protein allergy

Notes and References

1. Silverman . S.N. . Phillips . A.A. . Weiss . G.M. . Wilkes . E.B. . Eiler . J.M. . Sessions . A.L. . Practical considerations for amino acid isotope analysis . Organic Geochemistry . 2022 . 10.1016/j.orggeochem.2021.104345 . 164 . 104345. 2022OrGeo.16404345S . 245556854 . free .
2. 10.15227/orgsyn.010.0100 . Cox. G.J.. King. H. . L-Tryptophane . 10 . 100 . 1930 . Org. Synth..
3. 10.15227/orgsyn.018.0043. L-Histidine Monohydrochloride. Organic Syntheses. 18. 43. 1938. G. L.. Foster. D.. Shemin.
4. d-Arginine Hydrochloride. E.. Brand . M.. Sandberg. Org. Synth.. 1932. 12. 4. 10.15227/orgsyn.012.0004.
5. Kale . Prajyoti . Mishra . Anusha . Annapure . Uday S. . Development of vegan meat flavour: A review on sources and techniques . Future Foods . June 2022 . 5 . 100149 . 10.1016/j.fufo.2022.100149. free .
6. Sharp . Matthew . Shields . Kevin . Lowery . Ryan . Lane . Jason . Partl . Jeremy . Holmer . Chase . Minevich . Julie . Souza . Eduardo De . Wilson . Jacob . The effects of beef protein isolate and whey protein isolate supplementation on lean mass and strength in resistance trained individuals - a double blind, placebo controlled study . Journal of the International Society of Sports Nutrition . 21 September 2015 . 12 . Suppl 1 . P11 . 10.1186/1550-2783-12-S1-P11 . 4595383 . free .
7. https://www.fda.gov/downloads/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/UCM517122.pdf Labeling of Infant Formula: Guidance for Industry
8. Boyle RJ, Ierodiakonou D, Khan T, Chivinge J, Robinson Z, Geoghegan N, Jarrold K, Afxentiou T, Reeves T, Cunha S, Trivella M, Garcia-Larsen V, Leonardi-Bee J . Hydrolysed formula and risk of allergic or autoimmune disease: systematic review and meta-analysis . BMJ . 352 . i974 . March 2016 . 26956579 . 4783517 . 10.1136/bmj.i974 .
9. Cave . Nicholas J. . Hydrolyzed Protein Diets for Dogs and Cats . Veterinary Clinics of North America: Small Animal Practice . November 2006 . 36 . 6 . 1251–1268 . 10.1016/j.cvsm.2006.08.008 . 17085233 .