Prostaglandin F2alpha Explained

Prostaglandin F (PGF in prostanoid nomenclature), pharmaceutically termed dinoprost, is a naturally occurring prostaglandin used in medicine to induce labor and as an abortifacient.[1] Prostaglandins are lipids throughout the entire body that have a hormone-like function.[2] In pregnancy, PGF is medically used to sustain contracture and provoke myometrial ischemia to accelerate labor and prevent significant blood loss in labor.[3] Additionally, PGF has been linked to being naturally involved in the process of labor. It has been seen that there are higher levels of PGF in maternal fluid during labor when compared to at term.[4] This signifies that there is likely a biological use and significance to the production and secretion of PGF in labor. Prostaglandin is also used to treat uterine infections in domestic animals.

In domestic mammals, it is produced by the uterus when stimulated by oxytocin, in the event that there has been no implantation during the luteal phase. It acts on the corpus luteum to cause luteolysis, forming a corpus albicans and stopping the production of progesterone. Action of PGF is dependent on the number of receptors on the corpus luteum membrane.

The PGF isoform 8-iso-PGF was found in significantly increased amounts in patients with endometriosis, thus being a potential causative link in endometriosis-associated oxidative stress.[5]

Mechanism of action

PGF acts by binding to the prostaglandin F2α receptor. It is released in response to an increase in oxytocin levels in the uterus, and stimulates both luteolytic activity and the release of oxytocin.[6] Because PGF is linked with an increase in uterine oxytocin levels, there is evidence that PGF and oxytocin form a positive feedback loop to facilitate the degradation of the corpus luteum.[7] PGF and oxytocin also inhibit the production of progesterone, a hormone that facilitates corpus luteum development. Conversely, higher progesterone levels inhibit production of PGF and oxytocin, as the effects of the hormones are in opposition to each other. This is directly exhibited following ovulation when there is a spike of progesterone levels, and then as progesterone levels decrease, PGF levels will peak.[8]

Pharmaceutical Use

When injected into the body or amniotic sac, PGF can either induce labor or cause an abortion depending on the concentration used. In small doses (1–4 mg/day), PGF acts to stimulate uterine muscle contractions, which aids in the birth process. However, during the first trimester and in higher concentrations (40 mg/day),[9] PGF can cause an abortion by degrading the corpus luteum, which normally acts to maintain pregnancy via the production of progesterone. Since the fetus is not viable outside the womb by this time, the lack of progesterone leads to the shedding of the uterine lining and the death of the fetus. However, this process is not fully understood.

Pyometra and uterine infections

Lutalyse is used for the treatment of pyometra in domestic dogs and cats.[10] The drug is also administered to dairy cows in order to reduce uterine infections.[11]

Synthesis

Industrial Synthesis

In 2012 a concise and highly stereoselective total synthesis of PGF was described.[12] The synthesis requires only seven steps, a huge improvement on the original 17-steps synthesis of Corey and Cheng,[13] and uses 2,5-dimethoxytetrahydrofuran as a starting reagent, with S-proline as an asymmetric catalyst.

In 2019, a more effective and stereoselective synthesis was described.[14] The synthesis requires 5 steps to get to the intermediate which then undergoes a cross-metathesis reaction to install the E-alkene. Then, a Wittig reaction is performed to install the Z-alkene. Finally, the protecting groups are removed with acid.

In the body PGF is synthesized in several distinct steps. First, phospholipase A2 (PLA2) facilitates the conversion of phospholipids to arachidonic acid, the framework from which all prostaglandins are formed.[15] Arachidonic acid then reacts with two cyclooxygenase (COX) receptors, COX-1 and COX-2, or PGH synthase to form prostaglandin H2, an intermediate. Lastly, the compound reacts with aldose reductase or prostaglandin F synthase to form PGF.

Analogues

The following medications are analogues of prostaglandin F:

Notes and References

  1. Book: The Merck index: an encyclopedia of chemicals, drugs, and biologicals.. 2013. Royal Society of Chemistry. O'Neil MJ . 978-1849736701. 15th. Cambridge, UK. 824530529.
  2. Web site: Prostaglandin. September 28, 2022. Britannica. November 6, 2022.
  3. Kerekes L, Domokos N . The effect of prostaglandin F2 alpha on third stage labor . Prostaglandins . 18 . 1 . 161–166 . July 1979 . 392622 . 10.1016/S0090-6980(79)80034-9 .
  4. Sahmay S, Coke A, Hekim N, Atasu T . Maternal, umbilical, uterine and amniotic prostaglandin E and F2 alpha levels in labour . The Journal of International Medical Research . 16 . 4 . 280–285 . 1988 . 3169373 . 10.1177/030006058801600405 . 73028858 .
  5. Sharma I, Dhaliwal LK, Saha SC, Sangwan S, Dhawan V . Role of 8-iso-prostaglandin F2alpha and 25-hydroxycholesterol in the pathophysiology of endometriosis . Fertility and Sterility . 94 . 1 . 63–70 . June 2010 . 19324352 . 10.1016/j.fertnstert.2009.01.141 . free .
  6. Samuelsson B, Goldyne M, Granström E, Hamberg M, Hammarström S, Malmsten C . Prostaglandins and thromboxanes . Annual Review of Biochemistry . 47 . 997–1029 . 1978 . 209733 . 10.1146/annurev.bi.47.070178.005025 .
  7. Hooper SB, Watkins WB, Thorburn GD . Oxytocin, oxytocin-associated neurophysin, and prostaglandin F2 alpha concentrations in the utero-ovarian vein of pregnant and nonpregnant sheep . Endocrinology . 119 . 6 . 2590–2597 . December 1986 . 3465529 . 10.1210/endo-119-6-2590 .
  8. Downie J, Poyser NL, Wunderlich M . Levels of prostaglandins in human endometrium during the normal menstrual cycle . The Journal of Physiology . 236 . 2 . 465–472 . January 1974 . 16992446 . 1350813 . 10.1113/jphysiol.1974.sp010446 .
  9. Web site: Dinoprost tromethamine Injection Advanced Patient Information. 2016. Truvn Health Analytics Inc.. November 2, 2017.
  10. Davidson AP, Feldman EC, Nelson RW . Treatment of pyometra in cats, using prostaglandin F2 alpha: 21 cases (1982-1990) . Journal of the American Veterinary Medical Association . 200 . 6 . 825–828 . March 1992 . 1568932 . 2 December 2021 . National Library of Medicine . 10.2460/javma.1992.200.06.825 .
  11. Web site: Menino A . Evaluation of Single Lutalyse Injection Protocol to Reduce Uterine Infections and Improve Reproductive Efficiency in Postpartum Dairy Cows. . USDA . OREGON STATE UNIVERSITY . 2 December 2021.
  12. Coulthard G, Erb W, Aggarwal VK . Stereocontrolled organocatalytic synthesis of prostaglandin PGF2α in seven steps . Nature . 489 . 7415 . 278–281 . September 2012 . 22895192 . 10.1038/nature11411 . 205230275 . 2012Natur.489..278C .
  13. Book: The Logic of Chemical Synthesis . Corey EJ, Cheng XM . Wiley . 1995 .
  14. Kim T, Lee SI, Kim S, Shim SY, Ryu DH . Total synthesis of PGF2α and 6,15-diketo-PGF1α and formal synthesis of 6-keto-PGF1α via three-component coupling.. Tetrahedron . 75 . 42 . 130593 . 2019 . 10.1016/j.tet.2019.130593. 203131829.
  15. Fortier MA, Krishnaswamy K, Danyod G, Boucher-Kovalik S, Chapdalaine P . A postgenomic integrated view of prostaglandins in reproduction: implications for other body systems . Journal of Physiology and Pharmacology . 59 . 65–89 . August 2008 . 18802217 .