Prostaglandin-E2 9-reductase explained

prostaglandin-E2 9-reductase
Ec Number:	1.1.1.189
Cas Number:	42613-35-4
Go Code:	0050221

In enzymology, a prostaglandin-E₂ 9-reductase is an enzyme that catalyzes the chemical reaction

(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP⁺

\rightleftharpoons (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H⁺

Thus, the two substrates of this enzyme are (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate and NADP⁺, whereas its 3 products are (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate:NADP⁺ 9-oxidoreductase. Other names in common use include PGE₂-9-OR, reductase, 15-hydroxy-9-oxoprostaglandin, 9-keto-prostaglandin E₂ reductase, 9-ketoprostaglandin reductase, PGE-9-ketoreductase, PGE₂ 9-oxoreductase, PGE₂ reductase-9-ketoreductase, prostaglandin 9-ketoreductase, prostaglandin E 9-ketoreductase, and prostaglandin E₂ reductase-9-oxoreductase. This enzyme participates in arachidonic acid metabolism.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes,, and .

References

• Lee SC, Levine L . 1975 . Purification and regulatory properties of chicken heart prostaglandin E 9-ketoreductase . J. Biol. Chem. . 250 . 4549–55 . 166995 . 12 . 10.1016/S0021-9258(19)41336-7 . free .
• Schlegel W, Kruger S, Korte K . 1984 . Purification of prostaglandin E₂ reductase-9-oxoreductase from human decidua vera . FEBS Lett. . 171 . 141–4 . 6586494 . 10.1016/0014-5793(84)80475-5 . 1 . 25623206 . free .
• Book: Tai HH, Yuan B . 1982 . Purification and assay of 9-hydroxyprostaglandin dehydrogenase from rat kidney . Methods Enzymol. . 86 . 113–7 . 7132747 . 10.1016/0076-6879(82)86178-8 . 978-0-12-181986-6 .
• Watkins JD, Jarabak J . 1985 . The effect of NaCl intake on 9-ketoprostaglandin reductase activity in the rabbit kidney . Prostaglandins . 30 . 335–49 . 3901124 . 10.1016/0090-6980(85)90196-0 . 2 .