Proline 3-hydroxylase explained

Proline 3-hydroxylase
Ec Number:	1.14.11.28

In enzymology, a proline 3-hydroxylase is an enzyme that catalyzes the chemical reaction

L-proline + 2-oxoglutarate + O₂

\rightleftharpoons cis-3-hydroxy-L-proline + succinate + CO₂

The 3 substrates of this enzyme are L-proline, 2-oxoglutarate, and O₂, whereas its 3 products are cis-3-hydroxy-L-proline, succinate, and CO₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with 2-oxoglutarate as one donor, and incorporation of one atom o oxygen into each donor. The systematic name of this enzyme class is L-proline,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating). This enzyme is also called P-3-H.

References

• Mori H, Shibasaki T, Uozaki Y, Ochiai K, Ozaki A . 1996 . Detection of Novel Proline 3-Hydroxylase Activities in Streptomyces and Bacillus spp. by Regio- and Stereospecific Hydroxylation of l-Proline . Appl. Environ. Microbiol. . 62 . 1903 - 1907 . 16535329 . 6 . 1388867 .
• Mori H, Shibasaki T, Yano K, Ozaki A . 1997 . Purification and cloning of a proline 3-hydroxylase, a novel enzyme which hydroxylates free L-proline to cis-3-hydroxy-L-proline . J. Bacteriol. . 179 . 5677 - 83 . 9294421 . 18 . 179453 .
• Clifton IJ, Hsueh LC, Baldwin JE, Harlos K, Schofield CJ . 2001 . Structure of proline 3-hydroxylase. Evolution of the family of 2-oxoglutarate dependent oxygenases . Eur. J. Biochem. . 268 . 6625 - 36 . 11737217 . 10.1046/j.0014-2956.2001.02617.x . 24 . free .