Procyclin Explained
| EP1 procyclin |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | Tb10.6k15.0020 |
| Entrezgene: | 3661797 |
| Refseqmrna: | XM_818154.1 |
| Refseqprotein: | XP_823247.1 |
| Uniprot: | Q389V1 |
| Chromosome: | 10 |
| Entrezchromosome: | NC_007283.1 |
| Genloc Start: | 2481013 |
| Genloc End: | 2481566 |
| Surface protein EP1-2 procyclin |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | EP1-2 |
| Uniprot: | Q7KG35 |
| EP2 procyclin |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | Tb10.6k15.0030 |
| Entrezgene: | 3661534 |
| Refseqmrna: | XM_818153.1 |
| Refseqprotein: | XP_823246.1 |
| Uniprot: | Q389V2 |
| Chromosome: | 10 |
| Entrezchromosome: | NC_007283.1 |
| Genloc Start: | 2479700 |
| Genloc End: | 2480205 |
| Surface protein EP2-1 procyclin |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | EP2-1 |
| Uniprot: | Q95PJ2 |
| Procyclin EP3 |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | EP3 |
| Uniprot: | Q86MA3 |
| EP3-2 procyclin |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | Tb927.6.520 |
| Entrezgene: | 3657688 |
| Refseqmrna: | XM_840082.1 |
| Refseqprotein: | XP_845175.1 |
| Uniprot: | Q581F6 |
| Chromosome: | 6 |
| Entrezchromosome: | NC_007279.1 |
| Genloc Start: | 227056 |
| Genloc End: | 227561 |
| Surface protein EP3-3 procyclin |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | EP3-3 |
| Uniprot: | Q95NW2 |
| Surface protein EP3-4 procyclin |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | EP3-4 |
| Uniprot: | Q95PJ3 |
| GPEET2 procyclin precursor |
| Organism: | Trypanosoma brucei |
| Taxid: | 999953 |
| Symbol: | Tb927.6.510, Tb06.28F21.90 |
| Entrezgene: | 3657686 |
| Refseqmrna: | XM_840081.1 |
| Refseqprotein: | XP_845174.1 |
| Uniprot: | Q581F9 |
| Chromosome: | 6 |
| Entrezchromosome: | NC_007279.1 |
| Genloc Start: | 225784 |
| Genloc End: | 226232 |
Procyclins also known as procyclic acidic repetitive proteins or PARP[1] are proteins developed in the surface coating of Trypanosoma brucei parasites while in their tsetse fly vector.[2] The cell surface of the bloodstream form features a dense coat of variable surface glycoproteins (VSGs) which is replaced by an equally dense coat of procyclins when the parasite differentiates into the procylic form in the tsetse fly midgut.
There are six or seven procyclin genes that encode unusual proteins with extensive tandem repeat units of glutamic acid (E) and proline (P), referred to as EP repeats (EP1, EP1-2, EP2, EP2-1, EP3, EP3-2, EP3-4), and two genes that encode proteins with internal pentapeptide GPEET repeats (GPEET2).[3]
EP1 is a 141 amino acids protein and EP2 is a 129 AA protein. Both proteins have their coding genes situated on chromosome 10. GPEET2 is a 114 AA protein and EP3-2 is 123 AA protein with genes situated on chromosome 6.
See also
Notes and References
- Rudenko G, Le Blancq S, Smith J, Lee MG, Rattray A, Van der Ploeg LH . Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei . Mol. Cell. Biol. . 10 . 7 . 3492–504 . July 1990 . 1694012 . 360784 . 10.1128/MCB.10.7.3492 .
- Acosta-Serrano A, Vassella E, Liniger M, Kunz Renggli C, Brun R, Roditi I, Englund PT. Paul Englund . The surface coat of procyclic Trypanosoma brucei: programmed expression and proteolytic cleavage of procyclin in the tsetse fly . Proc. Natl. Acad. Sci. U.S.A. . 98 . 4 . 1513–8 . February 2001 . 11171982 . 29288 . 10.1073/pnas.98.4.1513 . 2001PNAS...98.1513A . free .
- Bütikofer P, Ruepp S, Boschung M, Roditi I . 'GPEET' procyclin is the major surface protein of procyclic culture forms of Trypanosoma brucei brucei strain 427 . Biochem. J. . 326 . 2. 415–23 . September 1997 . 9291113 . 1218686 . 10.1042/bj3260415.
