Proclavaminate amidinohydrolase explained
| Proclavaminate amidinohydrolase |
| Ec Number: | 3.5.3.22 |
In enzymology, a proclavaminate amidinohydrolase is an enzyme that catalyzes the chemical reaction
amidinoproclavaminate + H2O
proclavaminate + urea
Thus, the two substrates of this enzyme are amidinoproclavaminate and H2O, whereas its two products are proclavaminate and urea.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is amidinoproclavaminate amidinohydrolase. Other names in common use include PAH, and proclavaminate amidino hydrolase. This enzyme participates in clavulanic acid biosynthesis.
References
- Salowe SP, Krol WJ, Iwata-Reuyl D, Townsend CA . 1991 . Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction . Biochemistry . 30 . 2281 - 92 . 1998687 . 10.1021/bi00222a034 . 8 .
- Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA . Solomon EI . Edward I. Solomon . 2001 . Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities . J. Am. Chem. Soc. . 123 . 7388 - 98 . 11472170 . 10.1021/ja004025+. 30 .
- Townsend CA . 2002 . New reactions in clavulanic acid biosynthesis . Curr. Opin. Chem. Biol. . 6 . 583 - 9 . 12413541 . 10.1016/S1367-5931(02)00392-7 . 5 .
- Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA . 1995 . Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis . J. Bacteriol. . 177 . 3714 - 20 . 7601835 . 13 . 177087 .
