Probabilistic genotyping is the use of statistical methods and mathematical algorithms in DNA Profiling. It may be used instead of manual methods in difficult situations, such as when a DNA sample is very small or includes a mixture of multiple individuals' DNA.[1] Probabilistic genotyping, unlike traditional methods, avoids the need for subjective judgment.[1] The reliability of the method has been questioned by some defense lawyers because the source code of some probabilistic genotyping programs is proprietary.[2]