Polynucleotide 5'-hydroxyl-kinase explained

ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase
Ec Number:	2.7.1.78
Cas Number:	37211-65-7
Go Code:	0046404

In enzymology, a polynucleotide 5'-hydroxyl-kinase is an enzyme that catalyzes the chemical reaction

ATP + 5'-dephospho-DNA

\rightleftharpoons ADP + 5'-phospho-DNA

Thus, the two substrates of this enzyme are ATP and 5'-dephospho-DNA, whereas its two products are ADP and 5'-phospho-DNA. Polynucleotide kinase is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ATP to the free hydroxyl end of the 5' DNA or RNA. The resulting product could be used to end-label DNA or RNA, or in ligation reactions.

Nomenclature

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as an acceptor. The systematic name of this enzyme class is ATP:5'-dephosphopolynucleotide 5'-phosphotransferase. Other names in common use include:

• ATP:5'-dephosphopolynucleotide 5'-phosphatase
• PNK
• polynucleotide 5'-hydroxyl kinase (phosphorylating),
• 5'-hydroxyl polynucleotide kinase,
• 5'-hydroxyl polyribonucleotide kinase,
• 5'-hydroxyl RNA kinase,
• DNA 5'-hydroxyl kinase,
• DNA kinase,
• polynucleotide kinase, and
• polynucleotide 5'-hydroxy-kinase.

References

• Novogrodsky A, Hurwitz J . 1966 . The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5'-hydroxyl termini . J. Biol. Chem. . 241 . 2923 - 32 . 4287929 . 12 .
• Novogrodsky A, Tal M, Traub A, Hurwitz J . 1966 . The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5'-hydroxyl polynucleotide kinase . J. Biol. Chem. . 241 . 2933 - 43 . 4287930 . 12 .

External links

• Vivo
• New England Biolabs' T4 PNK page