Polynucleotide 3'-phosphatase explained

polynucleotide 3′-phosphatase
Ec Number:	3.1.3.32
Cas Number:	37288-16-7
Go Code:	0046403

The enzyme polynucleotide 3′-phosphatase (EC 3.1.3.32) catalyzes the reaction

a 3′-phosphopolynucleotide + H₂O

\rightleftharpoons a polynucleotide + phosphate

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name is polynucleotide 3'-phosphohydrolase. Other names in common use include 2′(3′)-polynucleotidase, DNA 3′-phosphatase, deoxyribonucleate 3′-phosphatase, and 5′-polynucleotidekinase 3′-phosphatase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .

References

• Becker A, Hurwitz J . 1967 . The enzymatic cleavage of phosphate termini from polynucleotides . J. Biol. Chem. . 242 . 936 - 50 . 4289819 . 5 .