Polyenoic fatty acid isomerase explained

Polyenoic fatty acid isomerase
Ec Number:	5.3.3.13

In enzymology, a polyenoic fatty acid isomerase is an enzyme that catalyzes the chemical reaction

(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate

\rightleftharpoons (5Z,7E,9E,14Z,17Z)-icosapentaenoate

Hence, this enzyme has one substrate, (5Z,8Z,11Z,14Z,17Z)-icosapentaenoate, and one product, (5Z,7E,9E,14Z,17Z)-icosapentaenoate.

This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases transposing C=C bonds. The systematic name of this enzyme class is (5Z,8Z,11Z,14Z,17Z)-icosapentaenoate Delta8,11-Delta7,9-isomerase (trans-double-bond-forming). Other names in common use include PFI, eicosapentaenoate cis-Delta5,8,11,14,17-eicosapentaenoate, cis-Delta5-trans-Delta7,9-cis-Delta14,17 isomerase, (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate Delta8,11-Delta7,8-isomerase, (incorrect), (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate Delta8,11-Delta7,9-isomerase, and (trans-double-bond-forming).

References

• Wise ML, Hamberg M, Gerwick WH . William H. Gerwick . 1994 . Biosynthesis of conjugated triene-containing fatty acids by a novel isomerase from the red marine alga Ptilota filicina . Biochemistry . 33 . 15223 - 32 . 7803384 . 10.1021/bi00255a002 . 51 .
• Wise ML, Soderstrom K, Murray TF, Gerwick WH . William_H._Gerwick . 1996 . Synthesis and cannabinoid receptor binding activity of conjugated triene anandamide, a novel eicosanoid . Experientia . 52 . 88 - 92 . 8575565 . 10.1007/BF01922423 . 1 .
• Wise ML, Rossi J, Gerwick WH . William_H._Gerwick . 1997 . Characterization of the substrate binding site of polyenoic fatty acid isomerase, a novel enzyme from the marine alga Ptilota filicina . Biochemistry . 36 . 2985 - 92 . 9062129 . 10.1021/bi962158v . 10 .
• Zheng W, Wise ML, Wyrick A, Metz JG, Yuan L, Gerwick WH . William H. Gerwick . 2002 . Polyenoic fatty acid isomerase from the marine alga Ptilota filicina: protein characterization and functional expression of the cloned cDNA . Arch. Biochem. Biophys. . 401 . 11 - 20 . 12054482 . 10.1016/S0003-9861(02)00002-4 . 1 .