Pitrilysin Explained
| Pitrilysin |
| Ec Number: | 3.4.24.55 |
| Cas Number: | 81611-78-1 |
Pitrilysin (Escherichia coli protease III, protease Pi, proteinase Pi, PTR, Escherichia coli metalloproteinase Pi) is an enzyme.[1] [2] [3] [4] [5] This enzyme catalyses the following chemical reaction:
Preferential cleavage of -Tyr16- Leu- and -Phe25- Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as glucagon
This enzyme is present in bacteria Escherichia coli.
Notes and References
- Finch PW, Wilson RE, Brown K, Hickson ID, Emmerson PT . Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III . Nucleic Acids Research . 14 . 19 . 7695–703 . October 1986 . 3534791 . 311789 . 10.1093/nar/14.19.7695 .
- Affholter JA, Fried VA, Roth RA . Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III . Science . 242 . 4884 . 1415–8 . December 1988 . 3059494 . 10.1126/science.3059494 . 1988Sci...242.1415A .
- Becker AB, Roth RA . An unusual active site identified in a family of zinc metalloendopeptidases . Proceedings of the National Academy of Sciences of the United States of America . 89 . 9 . 3835–9 . May 1992 . 1570301 . 525585 . 10.1073/pnas.89.9.3835 . 1992PNAS...89.3835B . free .
- Ding L, Becker AB, Suzuki A, Roth RA . Comparison of the enzymatic and biochemical properties of human insulin-degrading enzyme and Escherichia coli protease III . The Journal of Biological Chemistry . 267 . 4 . 2414–20 . February 1992 . 10.1016/S0021-9258(18)45895-4 . 1733942 . free .
- Anastasi A, Knight CG, Barrett AJ . Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay . The Biochemical Journal . 290 (Pt 2) . 601–7 . March 1993 . 2 . 7680857 . 1132317 . 10.1042/bj2900601 .
