Phenylpyruvate tautomerase explained

phenylpyruvate tautomerase
Ec Number:	5.3.2.1
Cas Number:	9023-54-5
Go Code:	0050178

In enzymology, phenylpyruvate tautomerase or Macrophage migration inhibitory factor is an enzyme that catalyzes the chemical reaction

\rightleftharpoons enol-phenylpyruvate

Phenylpyruvate tautomerase has also been found to exhibit the same keto-enol tautomerism for 4-Hydroxyphenylpyruvic acid, which is structurally similar to phenylpyruvate but contains an additional hydroxyl moiety in the para position of the aromatic ring.^[1]

This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting keto- and enol-groups. The systematic name of this enzyme class is phenylpyruvate keto---enol-isomerase. This enzyme is also called phenylpyruvic keto-enol isomerase. This enzyme participates in tyrosine metabolism and phenylalanine metabolism.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes,,,,,, and .

References

• Blasi F, Fragomele F, Covelli I . 1969 . Thyroidal phenylpyruvate tautomerase. Isolation and characterization . J. Biol. Chem. . 244 . 4864 - 70 . 5824560 . 18 . 10.1016/S0021-9258(18)94283-3 . free .
• Book: Knox WE . 1955 . [38] Enzymes involved in conversion of tyrosine to acetoacetate . Methods Enzymol. . 2 . 289 - 295 . 10.1016/s0076-6879(55)02202-7 . 978-0-12-181802-9 .
• KNOX WE, PITT BM . 1957 . Enzymic catalysis of the keto-enol tautomerization of phenylpyruvic acids . J. Biol. Chem. . 225 . 675 - 88 . 13416270 . 2 . 10.1016/S0021-9258(18)64866-5 . free .

Notes and References

1. 9395080 . 417 . The macrophage migration inhibitory factor MIF is a phenylpyruvate tautomerase . FEBS Lett . 85–8 . Rosengren E, Aman P, Thelin S, Hansson C, Ahlfors S, Björk P, Jacobsson L, Rorsman H . 1997 . 1 . 10.1016/s0014-5793(97)01261-1 . 27800700 . 1997FEBSL.417...85R .